A light microscope is used to observe two structures that are 200 nm apart on the slide. What is the actual distance between the two structures when the magnification is changed from $$$\times 40$$$ to $$$\times 400$$$ ?

$2 \mu \mathrm{~m}$

$\quad 20 \mu \mathrm{~m}$

200 nm

2000 nm

Biology

IGCSE&ALevel

CAIE

Exam No：9700_w24_qp_12 Year：2024 Question No：1

Answer:

Knowledge points:

1.1.1 Candidates should be able to:make temporary preparations of cellular material suitable for viewing with a light microscope

1.1.2 Candidates should be able to:draw cells from microscope slides and photomicrographs

1.1.3 Candidates should be able to:calculate magnifications of images and actual sizes of specimens from drawings, photomicrographs and electron micrographs (scanning and transmission)

1.1.4 Candidates should be able to: use an eyepiece graticule and stage micrometer scale to make measurements and use the appropriate units, millimetre (mm), micrometre (µm) and nanometre (nm)

1.1.5 Candidates should be able to: define resolution and magnification and explain the differences between these terms, with reference to light microscopy and electron microscopy

A light microscope is used to observe two structures that are 200 nm apart on the slide. What is the actual distance between the two structures when the magnification is changed from $$\(\times 40\)$$ to $$\(\times 400\)$$ ?

Answer:

Knowledge points:

Solution:

A light microscope is used to observe two structures that are 200 nm apart on the slide. What is the actual distance between the two structures when the magnification is changed from $$\(\times 40\)$$ to $$\(\times 400\)$$ ?

Answer:

Knowledge points:

Solution:

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