A student carried out investigations at $$$\mathrm{pH} 1-8$$$ to look at the effect of pH on an enzyme-catalysed reaction. The optimum condition for this enzyme is the acidic environment of the stomach at $$$\mathrm{pH} 1-2$$$. The remaining substrate concentration was measured after five minutes at each different pH . Which graph shows the effect of increasing pH on substrate concentration remaining after five minutes?

Biology

IGCSE&ALevel

CAIE

Exam No：9700_w24_qp_11 Year：2024 Question No：12

Answer:

Knowledge points:

3.2.1.1 temperature

3.2.1.2 pH (using buffer solutions)

3.2.1.3 enzyme concentration

3.2.1.4 substrate concentration

3.2.1.5 inhibitor concentration

3.2.2 explain that the maximum rate of reaction (Vmax) is used to derive the Michaelis-Menten constant (Km) which is used to compare the affinity of different enzymes for their substrates

3.2.3 explain the effects of inhibitors, both competitive and non- competitive, on the rate of enzyme activity

3.2.4 investigate the difference in activity between an enzyme immobilised in alginate and the same enzyme free in solution, and state the advantages of using immobilised enzymes

Answer:

Knowledge points:

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