A student investigated the effect of gibberellin(GA)and abscisic acid(ABA)on the germination of wheat grains. The student found out the following information. -The molecular mass of GA is 346 g . -The molecular mass of ABA is 264 g . -The range of concentrations at which GA is active in plant tissues is from $$\(1 \times 10^{-6}\)$$ to $$\(1 \times 10^{-5} \mathrm{moldm}^{-3}\)$$ . -The range of concentrations at which ABA is active in plant tissues is from $$\(1 \times 10^{-6}\)$$ to $$\(1 \times 10^{-4} \mathrm{moldm}^{-3}\)$$ . -Newly formed wheat grains contain a high concentration of ABA which decreases over time. -When the concentration of ABA falls low enough the GA is able to promote germination. -Wheat germinates best at temperatures from $$\(10^{\circ} \mathrm{C}\)$$ to $$\(25^{\circ} \mathrm{C}\)$$ and without light. Fig. 1.1 shows the germination and early growth of wheat. To test the effects on germination of adding $$\(A B A\)$$ ,the student started by soaking wheat grains for 24 hours in a solution of $$\(1 \times 10^{-6} \mathrm{~mol}^{-3} \mathrm{GA}\)$$ .These were then split into several groups of 50 grains and a different concentration of ABA was added to each group. The student made a stock solution of ABA with a concentration of $$\(1 \times 10^{-3} \mathrm{~mol} \mathrm{dm}^{-3}\)$$ which was then diluted to make the solutions needed for the investigation. (a)(i)Using the information about ABA above,describe how the student made a stock solution of $$\(1 \times 10^{-3} \mathrm{~mol} \mathrm{dm}^{-3} \mathrm{ABA}\)$$ . . . . (ii) The student used the stock solution of $$\(1 \times 10^{-3} \mathrm{~mol} \mathrm{dm}^{-3} \mathrm{ABA}\)$$ to prepare a range of dilutions from $$\(1 \times 10^{-4} \mathrm{~mol} \mathrm{dm}^{-3}\)$$ to $$\(1 \times 10^{-7} \mathrm{~mol} \mathrm{dm}^{-3}\)$$ to use in their investigation. Each dilution had a volume of $$\(100 \mathrm{~cm}^{3}\)$$. Describe how the student prepared these dilutions. . . .

Biology
IGCSE&ALevel
CAIE
Exam No:9700_s25_qp_54 Year:2025 Question No:1(a)

Answer:



Knowledge points:

15.2.1 describe the rapid response of the Venus fly trap to stimulation of hairs on the lobes of modified leaves and explain how the closure of the trap is achieved
15.2.2 explain the role of auxin in elongation growth by stimulating proton pumping to acidify cell walls
15.2.3 describe the role of gibberellin in the germination of barley (see 16.3.4)
2.3.1 describe and draw the general structure of an amino acid and the formation and breakage of a peptide bond
2.3.2 explain the meaning of the terms primary structure, secondary structure, tertiary structure and quaternary structure of proteins
2.3.3.1 hydrophobic interactions
2.3.3.2 hydrogen bonding
2.3.3.3 ionic bonding
2.3.3.4 covalent bonding, including disulfide bonds
2.3.4 state that globular proteins are generally soluble and have physiological roles and fibrous proteins are generally insoluble and have structural roles
2.3.5 describe the structure of a molecule of haemoglobin as an example of a globular protein, including the formation of its quaternary structure from two alpha (α) chains (α–globin), two beta (β) chains (β–globin) and a haem group
2.3.6 relate the structure of haemoglobin to its function, including the importance of iron in the haem group
2.3.7 describe the structure of a molecule of collagen as an example of a fibrous protein, and the arrangement of collagen molecules to form collagen fibres
2.3.8 relate the structures of collagen molecules and collagen fibres to their function

Solution:

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