An eyepiece graticule can be calibrated using a stage micrometer. What is the correct reason why an eyepiece graticule is calibrated?

A.
An eyepiece graticule can be used to make measurements.
B.
An eyepiece graticule is magnified by the objective lens.
C.
An eyepiece graticule magnifies the specimen.
D.
An eyepiece graticule makes comparisons.
Biology
IGCSE&ALevel
CAIE
Exam No:9700_s16_qp_13 Year:2016 Question No:1

Answer:

A

Knowledge points:

1.1.1 Candidates should be able to:make temporary preparations of cellular material suitable for viewing with a light microscope
1.1.2 Candidates should be able to:draw cells from microscope slides and photomicrographs
1.1.3 Candidates should be able to:calculate magnifications of images and actual sizes of specimens from drawings, photomicrographs and electron micrographs (scanning and transmission)
1.1.4 Candidates should be able to: use an eyepiece graticule and stage micrometer scale to make measurements and use the appropriate units, millimetre (mm), micrometre (µm) and nanometre (nm)
1.1.5 Candidates should be able to: define resolution and magnification and explain the differences between these terms, with reference to light microscopy and electron microscopy

Solution:

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