An investigation was carried out to see if compound $$\(X\)$$ could improve the thermostability of an enzyme. Thermostable enzymes will function well at high temperatures. The results of the investigation are shown.

A.
B.
C.
D.
Biology
IGCSE&ALevel
CAIE
Exam No:9700_s25_qp_14 Year:2025 Question No:14

Answer:

C

Knowledge points:

3.2.1.1 temperature
3.2.1.2 pH (using buffer solutions)
3.2.1.3 enzyme concentration
3.2.1.4 substrate concentration
3.2.1.5 inhibitor concentration
3.2.2 explain that the maximum rate of reaction (Vmax) is used to derive the Michaelis-Menten constant (Km) which is used to compare the affinity of different enzymes for their substrates
3.2.3 explain the effects of inhibitors, both competitive and non- competitive, on the rate of enzyme activity
3.2.4 investigate the difference in activity between an enzyme immobilised in alginate and the same enzyme free in solution, and state the advantages of using immobilised enzymes

Solution:

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