Many flowers produce a sweet solution called nectar. Bees provided with nectar use enzyme $$$Q$$$ to change the nectar into honey. After testing a sample of nectar for the presence of reducing sugar using standard laboratory reagents, the sample was blue. After testing a sample of honey in the same way, the sample was orange. Which conclusion about the reaction catalysed by enzyme Q is consistent with these results?

Biology

IGCSE&ALevel

CAIE

Exam No：9700_m24_qp_12 Year：2024 Question No：11

Answer:

Knowledge points:

3.1.1 state that enzymes are globular proteins that catalyse reactions inside cells (intracellular enzymes) or are secreted to catalyse reactions outside cells (extracellular enzymes)

3.1.2 explain the mode of action of enzymes in terms of an active site, enzyme–substrate complex, lowering of activation energy and enzyme specificity, including the lock-and-key hypothesis and the induced-fit hypothesis

3.1.3 investigate the progress of enzyme-catalysed reactions by measuring rates of formation of products using catalase and rates of disappearance of substrate using amylase

3.1.4 outline the use of a colorimeter for measuring the progress of enzyme-catalysed reactions that involve colour changes

3.2.1.1 temperature

3.2.1.2 pH (using buffer solutions)

3.2.1.3 enzyme concentration

3.2.1.4 substrate concentration

3.2.1.5 inhibitor concentration

3.2.2 explain that the maximum rate of reaction (Vmax) is used to derive the Michaelis-Menten constant (Km) which is used to compare the affinity of different enzymes for their substrates

3.2.3 explain the effects of inhibitors, both competitive and non- competitive, on the rate of enzyme activity

3.2.4 investigate the difference in activity between an enzyme immobilised in alginate and the same enzyme free in solution, and state the advantages of using immobilised enzymes

Answer:

Knowledge points:

Solution:

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