Phosphatases are enzymes that catalyse the removal of phosphate from other molecules, releasing inorganic phosphate ( $$\(\mathrm{P}_{\mathrm{i}}\)$$ ). A phosphatase can be extracted from mung bean seedlings, Vigna radiata. A student found a published method to extract phosphatase from mung bean seedlings. - Use a pestle and mortar to grind mung bean seedlings, with a small volume of distilled water, to make a paste. - Add distilled water to the paste to make a mixture with a total volume of $$\(50 \mathrm{~cm}^{3}\)$$. - Filter the mixture and put the filtrate into a clean centrifuge tube. - Centrifuge the filtrate until a solid pellet is formed, as shown in Fig. 1.2. - Pour the liquid extract containing phosphatase into a clean test-tube. Describe how the student could use the dilutions from (c) and a colorimeter to estimate the concentration of phenolphthalein in a reaction mixture. . . . .

Answer:

Knowledge points:

Solution: