Photosynthesis occurs in chloroplasts.Students investigated the effect of light intensity on the rate of photosynthesis in a chloroplast suspension. The blue dye DCPIP can be used to investigate photosynthesis.During photosynthesis,the dark-blue colour of the DCPIP changes and gradually disappears so that it becomes colourless. A chloroplast suspension that is made from spinach leaves has a green colour. The students used this method: Step 1 Prepare a chloroplast suspension from fresh spinach leaves. Step 2 Label one test-tube $$\(\mathbf{A}\)$$ and another test-tube $$\(\mathbf{B}\)$$ . Step 3 Put $$\(1 \mathrm{~cm}^{3}\)$$ of the green chloroplast suspension into test-tube $$\(\mathbf{A}\)$$ and into test-tube $$\(\mathbf{B}\)$$ . Step 4 Put test-tubes $$\(\mathbf{A}\)$$ and $$\(\mathbf{B}\)$$ into an ice-cold water-bath for two minutes. Step 5 Put $$\(5 \mathrm{~cm}^{3}\)$$ of the blue DCPIP solution into test-tube A.Put a test-tube rack 10 cm from a bright light.Put test-tube $$\(\mathbf{A}\)$$ into the test-tube rack. Step 6 Turn on the light and start the stop-clock. Step 7 Measure the time taken for the blue liquid in test-tube $$\(\mathbf{A}\)$$ to return to a green colour. Step 8 Reset the stop-clock to zero. Step 9 Move the test-tube rack so that it is 30 cm from the bright light. Step 10 Put $$\(5 \mathrm{~cm}^{3}\)$$ of the blue DCPIP solution into test-tube B. Put test-tube B into the test-tube rack and start the stop-clock. Step 11 Measure the time taken for the blue liquid in test-tube $$\(\mathbf{B}\)$$ to return to a green colour. The times measured in step 7 and step 11 are shown in Fig.1.1. time recorded for test-tube $$\(\mathbf{A}\)$$ time recorded for test-tube B Fig. 1.1 * 0000800000003 * (c)Fig. 1.2 is a photograph of a seed from a dandelion plant,Taraxacum officinale. Fig. 1.2 (i) Draw a large diagram of the whole dandelion seed shown in Fig. 1.2. (ii) Line $$\(\mathbf{P Q}\)$$ in Fig. 1.2 represents the length of the achene of the dandelion seed. Measure the length of line $$\(P Q\)$$ in Fig. 1.2. length of $$\(P \mathbf{Q}\)$$ ........................................... mm Calculate the actual length of the achene, using the formula and your measurement. $$\[ \text { magnification }=\frac{\text { length of line } P Q \text { in Fig. } 1.2}{\text { actual length of the achene }} \]$$ Give your answer to two significant figures. Space for working. ..................................................... . mm

Biology
IGCSE&ALevel
CAIE
Exam No:0610_w24_qp_62 Year:2024 Question No:1(c)

Answer:





Knowledge points:

16.3.1 Identify and draw, using a hand lens if necessary, the sepals, petals, stamens, filaments and anthers, carpels, style, stigma, ovary and ovules, of an insect-pollinated flower
16.3.10 Investigate and state the environmental conditions that affect germination of seeds, limited to the requirement for water, oxygen and a suitable temperature
16.3.11 Define self-pollination as the transfer of pollen grains from the anther of a flower to the stigma of the same flower or different flower on the same plant
16.3.12 Define cross-pollination as transfer of pollen grains from the anther of a flower to the stigma of a flower on a different plant of the same species
16.3.13 Discuss the implications to a species of self-pollination and cross-pollination in terms of variation, capacity to respond to changes in the environment and reliance on pollinators
16.3.14 Describe the growth of the pollen tube and its entry into the ovule followed by fertilisation (details of production of endosperm and development are not required)
16.3.2 State the functions of the sepals, petals, anthers, stigmas and ovaries
16.3.3 Use a hand lens to identify and describe the anthers and stigmas of a wind-pollinated flower
16.3.4 Distinguish between the pollen grains of insect-pollinated and wind-pollinated flowers
16.3.5 Define pollination as the transfer of pollen grains from the anther to the stigma
16.3.6  Define pollination as the transfer of pollen grains from the anther to the stigma
16.3.7  Define pollination as the transfer of pollen grains from the anther to the stigma
16.3.8 State that fertilisation occurs when a pollen nucleus fuses with a nucleus in an ovule
16.3.9 Describe the structural adaptations of insect-pollinated and wind-pollinated flowers
2.3.1 Calculate magnification and size of biological specimens using millimetres as units
2.3.2 Calculate magnification and size of biological specimens using millimetres and micrometres as units

Solution:

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