Squid is an animal that is eaten in many parts of the world. The body of the squid is used as meat because it contains a lot of protein. The photograph shows some squid meat. (Source: © Ilia Nesolenyi/Alamy Stock Photo) The meat from squid is quite tough and is often treated with enzymes to break down the protein. Some fruits contain enzymes that can break down protein. Pineapples contain the enzyme bromelain and unripe papayas contain the enzyme papain. A study compared the effects of two different concentrations of these two enzymes on the meat from squid. The flowchart shows some of the steps in the method used in this study. The graph shows the pH of the solutions measured at step 4. (i) The $$\(0.001 \%\)$$ enzyme solution was prepared by diluting the $$\(0.004 \%\)$$ enzyme solution. Calculate the volume of the $$\(0.004 \%\)$$ enzyme solution needed to make $$\(50 \mathrm{~cm}^{3}\)$$ of $$\(0.001 \%\)$$ enzyme solution. (1) Answer .......................................................... $$\(\mathrm{cm}^{3}\)$$ (ii) State two conclusions that can be made from the data shown in the graph. (2) ........................................................................................................................................................................................................................................................................... .. ................................................................................................................................................................................................................................................................................ ................................................................................................................................................................................................................................................................................ ........................................................................................................................................................................................................................................................................... .. ................................................................................................................................................................................................................................................................................ ................................................................................................................................................................................................................................................................................
Exam No：wbi13-01-que-20240523 Year：2024 Question No：IGCSE&ALevelBiology2024AS0597
Answer:
- \(12.5\left(\mathrm{~cm}^{3}\right)\)
A description that includes two of the following points:
- both enzymes decrease the pH (more than in the control) / at higher concentration the decrease in pH is more than at lower concentration / decrease in pH in bromelain more (than in papain) (1)
- bromelain is more effective at breaking down the protein (than papain) (1)
- at the higher concentration both enzymes are more effective at breaking down the protein (than at lower concentration) (1)
A description that includes two of the following points:
- both enzymes decrease the pH (more than in the control) / at higher concentration the decrease in pH is more than at lower concentration / decrease in pH in bromelain more (than in papain) (1)
- bromelain is more effective at breaking down the protein (than papain) (1)
- at the higher concentration both enzymes are more effective at breaking down the protein (than at lower concentration) (1)
Knowledge points:
CH2 – Membranes, Proteins, DNA and Gene Expression
Solution:
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