Yeast cells contain enzymes that catalyse the breakdown of sugars, such as glucose and sucrose, to produce ethanol and carbon dioxide. As the sugars are broken down, the yeast cells in a suspension will sink slowly to the bottom of the container, forming a sediment with a clear liquid above. This process is called sedimentation. You will investigate the effect of different concentrations of ethanol on the sedimentation of yeast cells. Test-tube $$\(\mathbf{R}\)$$ in Fig. 1.1 shows how a test-tube will be set up at the start of the investigation. (i) Decide what you expect the contents of the test-tube to look like after sedimentation. On test-tube $$\(\mathbf{S}\)$$ in Fig. 1.1, draw: - lines to show the layers that you expect to see after sedimentation - a label line and label to identify the sediment. You are provided with the materials shown in Table 1.1. You will investigate the effect of different concentrations of ethanol on the sedimentation of yeast cells. You will need to: - prepare different concentrations of ethanol - measure the height of the yeast sediment in each concentration of ethanol every four minutes. You will use proportional dilution to make different concentrations of ethanol. You will prepare $$\(10 \mathrm{~cm}^{3}\)$$ of each concentration, using $$\(\mathbf{E}\)$$ and $$\(\mathbf{W}\)$$. Table 1.2 shows how to prepare two of the concentrations you will use. Decide which other concentrations of ethanol you will use. (ii) Complete Table 1.2 to show how you will prepare the concentrations of ethanol you will use. Carry out step 1 to step 15 . step 1 In the beakers provided, prepare the concentrations of ethanol as shown in Table 1.2. step 2 Label one test-tube with the label $$\(\mathbf{C}\)$$. step 3 Label the other test-tubes with the concentrations of ethanol prepared in step 1. step 4 Put $$\(1 \mathrm{~cm}^{3}\)$$ of $$\(\mathbf{A}\)$$ into each of the test-tubes labelled in step 2 and step 3. step 5 Put $$\(7 \mathrm{~cm}^{3}\)$$ of $$\(\mathbf{W}\)$$ into the test-tube labelled $$\(\mathbf{C}\)$$. step 6 Put $$\(7 \mathrm{~cm}^{3}\)$$ of the $$\(100 \%\)$$ ethanol, E, into the test-tube labelled $$\(100 \%\)$$. step 7 Repeat step 6 for each of the other concentrations you prepared in step 1. step 8 Put $$\(100 \mathrm{~cm}^{3}\)$$ of $$\(\mathbf{W}\)$$ into the beaker labelled $$\(\mathbf{Y}\)$$ and stir until all of the dried yeast forms a suspension. step 9 Stir the yeast cell suspension and put $$\(7 \mathrm{~cm}^{3}\)$$ of the yeast cell suspension into the test-tube labelled $$\(100 \%\)$$. step 10 Repeat step 9 for each of the other labelled test-tubes, including $$\(\mathbf{C}\)$$. step 11 Put a clean bung into one of the test-tubes and invert the test-tube to mix the contents. step 12 Repeat step 11 for all the other test-tubes. step 13 Start timing and immediately measure the height of the sediment in each test-tube. These are the results at the start ( 0 minutes). Record your results in (a)(iii). step 14 After 4 minutes, measure the height of the sediment in each test-tube. Record your results in (a)(iii). step 15 Repeat step 14 so that results are recorded every 4 minutes until the final results are recorded at 20 minutes. (iii) Record your results in an appropriate table. (iv) Using your results in (a)(iii), state one conclusion that can be made about the effect of the concentration of ethanol on the sedimentation of yeast cells. ....................................................................................................................................... . ....................................................................................................................................... . ....................................................................................................................................... . ................................................................................................................................. (v) A student suggested the hypothesis: ethanol is needed for sedimentation of yeast cells to occur. Using your results in (a)(iii), state whether you support or reject this hypothesis. Explain how your results provide evidence for this decision. support or reject ........................................... explanation .................................................................................................................... ....................................................................................................................................... . ....................................................................................................................................... . (vi) Using the results at 20 minutes, state the concentration, or concentrations, of ethanol that caused the most sedimentation. ....................................................................................................................................... . Suggest how you could modify this procedure to obtain a more accurate estimate of the concentration that causes the most sedimentation. modification ................................................................................................................... .. ....................................................................................................................................... . ....................................................................................................................................... . ....................................................................................................................................... . ....................................................................................................................................... . ....................................................................................................................................... . (vii) Identify the main source of error in the investigation into the effect of different concentrations of ethanol on the sedimentation of yeast cells. ....................................................................................................................................... . ................................................................................................................................. (viii) Another possible source of error, when carrying out step 6 of the investigation, is shown in Table 1.3. Complete Table 1.3 by stating whether the type of error is systematic or random and the effect the error may have on the trend seen in the results.

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